In 63 BCE, Pompey made Judea a part of the Roman Republic. Beit She’an was refounded and rebuilt by Gabinius. The town center shifted from the summit of the mound, or tell, to its slopes. Scythopolis prospered and became the leading city of the Decapolis, the only one west of the Jordan River.

The city flourished under the “Pax Romana”, as evidenced by high-level urban planning and extensive construction, including the best preserved Roman theatre of ancient Samaria, as well as a hippodrome, a cardo and other trademarks of the Roman influence. Mount Gilboa, 7 km away, provided dark basalt blocks, as well as water (via an aqueduct) to the town. Beit She’an is said to have sided with the Romans during the early phase of the First Jewish–Roman War in 66 CE. Excavations have focused less on the Roman period ruins, so not much is known about this period. The Penn. University Museum excavation of the northern cemetery, however, did uncover significant finds. The Roman period tombs are of the loculus type: a rectangular rock-cut spacious chamber with smaller chambers (loculi) cut into its side.